Unique Point-of-Care Diagnostic for Detection of Respiratory Viruses

Focus: 

To develop a point-of-care diagnostic test for the influenza and paramyxovirus families

Anticipated Impact: 

An inexpensive test for the most common respiratory viruses that can be performed in 30 minutes in a primary health care environment

Abstract: 

Dr. Rose and his team are developing an innovative microfluidics-based platform for point-of-care molecular detection of a broad range of respiratory pathogens. Over 220 million people worldwide suffer from respiratory infections each year. This number is expected to increase by 10-20% annually over the next decade, creating demand for tests to differentiate between infectious diseases with similar symptoms, especially in low-resource settings. The proposed product, under development by Micronics, Inc. (Redmond, WA), is a microfluidics cartridge that will operate in a compact battery-operated instrument, permitting diagnostic assays to be performed rapidly in low-resource settings at minimal cost. All reagents for specimen processing, PCR-based target amplification, detection, and read-out are integrated into a disposable cartridge. A unique feature is Dr. Rose’s CODEHOP PCR primer technology, which permits broad detection of related viruses in a single assay. The goal of the proposed work is to develop a diagnostic platform to identify and discriminate the influenza and paramyxovirus types on the same disposable cartridge. The influenza and paramyxovirus families of respiratory viruses are responsible for seasonal high hospitalization rates, outpatient visits, and deaths in children and the elderly. A rapid, point-of-care molecular diagnostic platform would provide timely and accurate diagnosis to the treating physician, avoiding costly and disruptive treatments or public health measures, over-prescription of antibiotics, and increased spread of disease.

Collaborating organization:  Micronics, Inc. 

Respiratory Infection Diagnosis

Grant Update

Principal Investigator:
Timothy Rose
Grantee Organization:
Seattle Children's Research Institute
Grant Title:
Unique point-of-care diagnostic for detection of respiratory viruses
Grant Cohort and Year:
2011 Second Round Commercialization (04)
Grant Period:
07/01/2012 - 06/30/2013 (Completed)
Grant Amount:
$146,951
We have successfully accomplished the goals proposed for the last six months of the project and have finalized assays to detect and identify respiratory infections due to members of the paramyxovirus and influenza virus families.

Impact in Washington

Location of LSDF Grantee
Locations of Collaborations/Areas of Impact
Seattle
Redmond

Legislative Districts:
11, 34, 36, 37, 43, 46, 48

Health Impacts

Respiratory Infection Diagnosis

Apr 15, 2014
Puget Sound Business Journal