HF-PCR™ Point of Care HIV Viral Load Assay Development


To validate the performance of a new system for measuring HIV-1 levels in blood

Anticipated Impact: 

Simpler and more cost-effective diagnosis and clinical management of HIV-1/AIDS, as well as other diseases as the system's functionality is enhanced


Health-care providers rely on quantitative polymerase chain reaction (qPCR) tests to diagnose and treat numerous human diseases. These tests include HIV-1 viral load assays, which are performed multiple times per year on HIV patients on antiretroviral treatment in Washington and throughout the US. Current qPCR systems are used only in advanced research and large clinical laboratories. HeatFlow Technologies has developed HF-PCR®, a proprietary method for performing qPCR tests that is simpler and less expensive than current technologies. As such, HF-PCR is anticipated to not only serve the needs of current qPCR users, but also to make qPCR practical and cost-effective for smaller clinical laboratories and the point-of-care market. The LSDF grant will support assessment of the HF-PCR technology’s HIV-1 detection sensitivity. Although HIV-1 testing is the focus of the grant, the company anticipates developing additional tests later.

Collaborating organization: Seattle BioMed

HIV Diagnosis, Management

Grant Update

Principal Investigator:
Warren Dinges
Grantee Organization:
HeatFlow Technologies, Inc.
Grant Title:
HF-PCR (TM) Point of Care HIV Viral Load Assay Development
Grant Cohort and Year:
2014 Proof of Concept (03)
Grant Period:
12/01/2014 - 08/03/2015 (Completed)
Grant Amount:

Impact in Washington

Location of LSDF Grantee
Locations of Collaborations/Areas of Impact

Legislative Districts:
11, 34, 36, 37, 43, 46

Health Impacts

HIV Diagnosis, Management